Fig. 1
From: Focal adhesion assembly and cell migration require myoferlin in PDAC cell lines
Myoferlin expression correlates with expression of actin cytoskeleton gene sets in PDAC patients and myoferlin knockdown PDAC cells. (A) Heatmap and unsupervised clustering of a 7 gene metastatic signature in PDAC TCGA patients (n = 177). Gene expression values are z-score normalized. (B) Correlation between MYOF gene expression and average expression of 7 genes included in a metastatic signature in PDAC TCGA patients (n = 177). (C) Repartition of N0 (no lymph node infiltration), N1 (one lymph node infiltrated) and N1B (several lymph nodes infiltrated) PDAC TCGA patients according to high and low MYOF groups. Chi-square test. (D) GSEA results of differentially expressed genes (p < 0.05) in high and low MYOF groups. Genesets extracted gene ontology (GO—cellular component ontology). (E) RNAseq-based MYOF, ACTA2, ACTB, ACTG1 expression in PDAC TCGA cohort patients (n = 177) according to MYOF expression. Boxplot, unpaired Student’s t test. (F) RNAseq-based MYOF expression in Panc-1 cells transfected with irrelevant (Irrel.) or myoferlin specific (MYOF) siRNA. G GSEA results of differentially expressed genes in Panc-1 cells transfected with myoferlin specific (MYOF) siRNA. Genesets extracted gene ontology (GO—cellular component ontology).
