Fig. 2
From: Focal adhesion assembly and cell migration require myoferlin in PDAC cell lines
Myoferlin knockdown tampers cytoskeleton ultrastructure, disorganizes microfilaments. (A) Ultrastructure of Panc-1 cells transfected with irrelevant or myoferlin specific (MYOF#1, #2, #3) siRNA. Red dotted lines delimitate areas with very dense cytoskeletal elements. Scale bar = 2 µm. Black dashed lines delimitate areas represented at higher magnification. Scale bar = 0.7 µm. (B) Size analysis of cytoskeletal element diameter at 30,000× magnification. Fiber diameters (n = 37) were measured in MYOF#2 samples from 6 different cells. (C) Western blot analysis of Panc-1 total-cell lysates. One representative western blot of three independent experiments is shown HSC70 was used as loading control. (D) Actin filaments visualization in Panc-1 cells transfected with irrelevant or myoferlin specific (MYOF#1, #2, #3) siRNA. Yellow arrowhead pointed to F-actin accumulation at plasma membrane. Scale bar = 20 µm. (E) Number of actin filaments per cell (n = 12), cumulative length of filaments per cell (n = 12), maximal filament length per cell (n = 12), and number of filament branches per cell (n = 12) in Panc-1 cells transfected with irrelevant or myoferlin specific (MYOF#1, #2, #3) siRNA. Violin plot, one-way ANOVA (Dunnett’s test), p-value relative to control group (Irrel. siRNA).
