Fig. 3
From: Extracellular matrix stiffness modulates angiogenic properties of the retinal pigment epithelium

Stiffness-dependent localization of THBS1 in ARPE-19 cells grown on substrates with stiffnesses of 30 kPa, 80 kPa and TCP. IF images illustrating the localization of the nuclei/DNA (DAPI, blue), THBS1 (green), F-Actin (yellow), cell borders (ZO-1, red) and merged images. The images are represented as orthogonal projections created from a confocal stack of 17 individual images, providing a comprehensive view of the cells from their apical to basal extent.