Fig. 1
LED-RL inhibits keratinocyte proliferation. (A) Total cell counts in control and LED-RL treated HaCaTs (n = 3 technical repeats). The incubator (negative) control remained in the 37 °C humidified incubator with 5% CO2 throughout the treatment protocol. LED-RL and matched control treatment protocols were performed outside of the incubator. (B) Total cell counts of LED-RL treated human primary keratinocytes (n = 5 technical repeats). (C) Representative phase contrast images of primary keratinocytes 48 h after LED-RL treatment. (D) Total cell counts of primary keratinocytes pre- or untreated with IL-22 and treated with LED-RL at doses of 320 and 640 J/cm2 (n = 3 technical repeats). Student’s t-test was utilized to determine statistical significance between LED-RL samples and their respective temperature-matched control group (1 h to 320 J/cm2 LED-RL; 2 hours to 640 J/cm2 LED-RL). * denotes p < 0.05.
