Fig. 5
Changes in periodontal conditions in dogs with P. gulae before and after daily mastic (5%) gel treatment. (A) Experimental schedule. (B) Methyl mercaptan (ppb) and (C) hydrogen sulfide (ppb) levels in expired breath were significantly reduced after 30–40 days of mastic treatment. (D) Gingivitis scores were significantly improved compared to pre-treatment values. (E) Plaque index showed no significant change following mastic treatment. (F) PCR analysis revealed a significant reduction in P. gulae levels after treatment, with no change in the vehicle control group. (G) Hydrolysis of BANA, indicative of periodontal pathogen activity, was suppressed by mastic treatment compared to pre-treatment and vehicle control levels. Data are presented as mean ± 1 SEM; n = 30 for mastic, n = 10 for vehicle control; p < 0.05 (uncorrected Fisher’s LSD test). BANA, N-benzoyl-DL-arginine-2-naphthylamide; PCR, polymerase chain reaction.
