Fig. 3 | Scientific Reports

Fig. 3

From: Mechanism of Saikosaponin D in regulating ferroptosis in patient-derived lung adenocarcinoma organoids via upregulation of ATF3/CHOP/CHAC1 signaling

Fig. 3

Response of lung adenocarcinoma PDO and cells to SSD. (A) Bright field images of different concentrations of SSD acting on LUAD-90, scale bar: 200 μm. (B) CCK-8 detection of the viability of organoids after 96 h of SSD treatment of 10 LUAD PDOs with different concentrations. Compared with the control group (n = 3). (C) Dose-response curves of 10 PDOs after 96 h. Each PDO was measured three times each time(n = 3). (D) IC50 of SSD and DDP acting on PDO, respectively (n = 3). (E) Dose-response curves of SSD in normal lung organoids and tumor organoids in the same individual (LUAD-95), where the IC50 in normal lung organoids is 2.4 µM, while the IC50 in tumor organoids is 1.0 µM (n = 3). (F) Bright field images of SSD acting on normal lung organoids and lung cancer PDOs, scale bar: 100 μm. (Data are presented as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001). PDO, patient-derived organoids; SSD, saikosaponin D.

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