Fig. 4

Principal component analysis of normalized RNA-seq read counts between the genotypic and temperature-induced males on the first two principal components. PCA based on all genes (A), the meiosis toolkit consisting of 40 genes (B), and male sex determination genes (C) are presented. The different colours indicate (1) genotypic males (red) and (2) temperature-induced males (i) resulting from sex reversal across the temperature range at 22–31 °C, with males sampled at low temperature (male group 3 - green) and high temperature (male group 2 - blue), or (ii) resulting from sex reversal only at high temperature (male group 1 - purple).