Table 2 List of sex determination genes with their differential expression levels between genotypic and temperature-induced males. Positive log2 fold change (l2fc) indicates transcripts that were more abundant in genotypic diploid males. Negative log2 fold change indicates transcripts that were more abundant in temperature-induced triploid males. Bold indicates significant differences in gene expression level between genotypic and induced males (padj < 0.05). Asterisks indicate significant differences in gene expression level between genotypic and induced males (shown in bold): * padj < 0.05, ** padj < 0.01, *** padj < 0.001.
Ensembl ID | Gene name | Gene description | L2fc | padj |
|---|---|---|---|---|
ENSCARG00000021243 | Amh | Anti-Müllerian hormone | 1.46 | *** |
ENSCARG00000000448 | Cirbpa | Cold-inducible RNA-binding protein A | −0.61 | ** |
ENSCARG00000069389 | Cyp19a1a | Cytochrome P450 19 A1a | 2.63 | *** |
ENSCARG00000015111 | Dmrt1 | Doublesex and mab-3 related transcription factor 1 | 0.09 | |
ENSCARG00000031029 | Dmrt1 | Doublesex and mab-3 related transcription factor 1 | 0.15 | |
ENSCARG00000031374 | Dmrt2a | Doublesex and mab-3 related transcription factor 2a | 0.63 | |
ENSCARG00000015272 | Dmrt2a | Doublesex and mab-3 related transcription factor 2a | −0.04 | |
ENSCARG00000013496 | Dmrt2b | Doublesex and mab-3 related transcription factor 2b | 0.21 | |
ENSCARG00000028285 | Dmrt2b | Doublesex and mab-3 related transcription factor 2b | −0.78 | * |
ENSCARG00000019977 | Dmrta2 | Doublesex and mab-3 related transcription factor a2 | 2.05 | * |
ENSCARG00000003716 | Foxl2b | Forkhead box L2 | 0.05 | |
ENSCARG00000036645 | Gata4 | GATA-Binding Factor 4 | 0.65 | |
ENSCARG00000033885 | Gdf6a | Growth Differentiation Factor 6 | −0.41 | |
ENSCARG00000055889 | Gsdf | Gonadal soma-derived factor | 1.55 | *** |
ENSCARG00000015482 | Igf1 | Insulin-like growth factor | −0.89 | |
ENSCARG00000058304 | Lhx9 | LIM Homeobox 9 | 1.83 | *** |
ENSCARG00000046772 | Nr0b1 (Dax1) | Nuclear receptor 0 B1 | 0.05 | |
ENSCARG00000003021 | Rspo1 | R-spondin 1 | −0.44 | |
ENSCARG00000066647 | Rspo1 | R-spondin 1 | 0.42 | |
ENSCARG00000043864 | Sf1 (nr5a1a) | steroidogenic factor 1 | −0.66 | *** |
ENSCARG00000052578 | Sox4b | SRY-box 4b | −1.88 | *** |
ENSCARG00000050582 | Sox7 | SRY-box 7 | −2.01 | *** |
ENSCARG00000058624 | Sox8a | SRY-box 8a | 0.91 | ** |
ENSCARG00000033646 | Sox9b | SRY-box 9b | 0.67 | * |
ENSCARG00000021129 | Star | Steroidogenic acute regulatory protein | 0.95 | * |
ENSCARG00000046195 | Wt1a | Wilm’s tumor 1a | −0.76 | * |