Table 6 List of male determination genes with their differential expression levels between genotypic and induced males. Three different groups of temperature-induced males are considered (group 1: full sex reversal at 28–31 °C, group 2: sampled at 28–31 °C from the offspring of a female with high sex reversal proportion at 22 °C and full sex reversal at 28–31 °C, group 3: sampled at 22 °C from the offspring of a female with high sex reversal proportion at 22 °C and full reversal at 28–31 °C). Differences in expression are indicated by the log2fold change. Positive log2 fold change (l2fc) indicates transcripts that were more abundant in genotypic males. Negative log2 fold change indicates transcripts that were more abundant in temperature-induced males. Bold indicates the genes with significant differences in gene expression level between genotypic males and each group of induced males (padj < 0.05). Asterisks indicate significant differences in gene expression level between genotypic and induced males of a given group (padj < 0.05).
Gene name | Male group 1 | Male group 2 | Male group 3 |
|---|---|---|---|
Amh | 0.61 | 1.03* | 1.40* |
Cirbpa | −0.51 | −0.47 | −0.75* |
Cyp19a1a | 2.55* | 2.27* | 2.69* |
Dmrt1 | 0.22 | 0.14 | 0.12 |
Dmrt1 | −0.19 | −0.07 | 0.02 |
Dmrt2a | −0.75* | 1.20 | 1.55* |
Dmrt2a | 0.00 | 0.52 | 0.49 |
Dmrt2b | −0.60 | 0.98 | 1.26* |
Dmrt2b | −1.21* | −0.32 | −0.17 |
Dmrta2 | 0.21 | 0.31 | 0.42 |
Foxl2b | −0.47 | 0.13 | −0.25 |
Foxl2b | −0.47 | 0.13 | −0.25 |
Fshr | −0.23 | 0.69* | 0.90* |
Gata4 | 0.61 | 1.43* | 1.22* |
Gdf6a | −1.36* | −0.37 | −0.71 |
Gsdf | 0.92* | 1.52* | 1.49* |
Igf1 | −1.67* | −1.12* | −1.68* |
Lhx9 | 1.88* | 2.48* | 2.77* |
Nr0b1(Dax1) | −0.11 | 0.85 | 0.84* |
Rspo1 | −1.16* | −0.27 | −0.07 |
Rspo1 | −0.43 | 1.28 | 1.48* |
Sf1 (Nr5a1a) | −0.65* | −0.30 | −0.43 |
Sox8a | 0.88* | 1.52* | 1.56* |
Sox9b | 0.61 | 1.09* | 0.97* |
Star | 1.13* | 1.02 | 1.15* |
Wt1a | −0.88* | −0.54 | −0.09 |