Table 7 List of the target genes selected from RNA-seq analysed using RT-qPCR. Their respective primer sequences and amplicon size are indicated.

From: Differential expression of reproduction and sex-determining genes in the gonads of genotypic and temperature-induced males of gibel carp (Carassius gibelio)

Gene name

Full name

Gene ID

forward/reverse primers (5’->3’)

Amplicon size

Ccna2

Cyclin A2

ENSCARG00000044731

AATACAAGGGCTCAAAGTACCA

197

   

AAGATCTCACCATTTCCCCAAA

 

Fbxo5 (emi1)

F-box Protein 5

ENSCARG00000056526

GGAGATTGTGACTTAGTGAGCTGT

138

   

TAATGAGCCCATGAGGCGAC

 

Actr2b

Activin receptor type-2B

ENSCARG00000061469

CGTCAAGAAGGGATGCTGGT

166

   

AGGAGACTCCAGCACTGGTC

 

Cdc20

Cell division cycle protein 20 homolog

ENSCARG00000027165

CCCAGCAAAACACCAGGAAAG

164

   

GGTGCTGTAGTGGATGCGA

 

Ccnb1

Cyclin B1

ENSCARG00000028136

AACCTCAGCGCACAATCAGT

129

   

CAGGCGAGTGTTCCTTGAGA

 

Ccnb3

Cyclin B3

ENSCARG00000062532

GGACTGGAGAACCAGGATGG

109

   

CAATCTTGTGTGCATTTGTGATGT

 

Ccnf

Cyclin F

ENSCARG00000060407

TCACTGTGAAGCATTATTGTTGAA

75

   

ACAGTGGAGCACGCCC

 

Spag1a

Sperm associated antigen 1a

ENSCARG00000007149

CACACAAAGGCTTGAAGGACTAT

171

   

TTCATCCTTTATTGCCTAGATGTCA

 

Ddx52

Dexd-Box Helicase 52

ENSCARG00000010183

GATTCATGCGGATCGCACAC

194

   

CTCTTCCAGTTCGCCCGAT

 

Gadd45ba

Growth arrest and DNA-damage-inducible, Beta a

ENSCARG00000056928

ACTGCATCATAGTCACTAACCCT

159

   

TCATTGCATCAAAAGCATAGCAGT

 

Fbxo15

F-box protein 15

ENSCARG00000037330

GTGCCACTCAATTGTCCTGC

167

   

ATTCCCCACTGGTCCTTCCA

 

Tekt1

Tektin 1

ENSCARG00000062861

GATTGGCAGCAGTTGACAGAAAAA

105

   

CGAGCTCCTGAAATGCACCT

 

Kif26a

Kinesin family member 26a

ENSCARG00000043633

CTGTGCACGCTCCACCTCTT

184

   

GGTGTAGCTTTTGCCGAGGT

 

Itga4

Integrin subunit alpha 4

ENSCARG00000023759

TGATTTCATCACTACCCTCTAGACA

197

   

TCCAACCATCAGCCATGTGT

 

Sox7

Sry-box transcription factor 7

ENSCARG00000050582

CTGAGCAAGATGCTGGGAAAG

138

   

CTTCTTCCTACGAGGACGGT

 

Izumo

Izumo sperm-egg fusion protein 1

ENSCARG00000049815

GGAGAGCATCATCTCGAGGC

140

   

TCCCCGTCTTGTGACAGATTC

 
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