Fig. 5

Experimental validation of differential gene expression in mice. (a) Typical colon anatomy of mice in each group. (b) Changes in the weight of mice throughout the experiment. (two-way ANOVA). (c) H&E staining of colon tissue; scale bar, 100 μm. (d) Immunofluorescence staining was used to show MUC2 expression in the colon. (e) The relative expression of Serpine1, Pea15a, Casp4, and Casp1 in colon tissue were detected by real-time PCR.(n ≥ 6).*P < 0.05 vs. DSS group, **P < 0.01 vs. DSS group, ***P < 0.001 vs. DSS group, ****P < 0.0001 vs. DSS group.