Fig. 3 | Scientific Reports

Fig. 3

From: Identification of a novel real-time PCR reference gene panel for EA.hy926 endothelial cells in different growth States and with DHA treatment

Fig. 3

RT-qPCR results for endothelial marker genes normalized to various combinations of HKGs. Normalized expression results for genes a) CD36, b) CDH5, c) HIF1A, d) PCNA, e) PECAM1, and f) VWF. Each panel is organized into 2 rows with the top row left to right representing cHKGs individually or in the indicated combinations: 18 S, 18 S + ACTB, and 18 S + ACTB + HPRT1, and the bottom row left to right representing sHKGs individually or in the indicated combinations: CAPZB, CAPZB + FBXO7, and CAPZB + FBXO7 + YWHAZ. n = 6–8 per gene per growth state (a growth state is day 4 or day 10 with or without Matrigel (MG)). A bar with asterisks (*: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001) indicates significant differences between specified groups; a bar with a p value indicates a trend (p = 0.05 to 0.1) between specified groups.

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