Table 1 Advantages and disadvantages of the three analytical methods.
FA-Fluo | CGE-UV (PA 800 Plus device) | IP-RPLC | |
|---|---|---|---|
Advantages | • More sensitive (due to the use of fluorescence dye) | • No fluorescence bias | • Easy sample preparation (less laborious) |
• Capable of separating mRNA-LNP adducts from the main peak | |||
• Easy to use | • More flexible method than Fragment Analyzer | • Better resolution | |
• Deformulation not needed | |||
• Fastest analysis | • Capable of detecting mRNA dimer | • Less day-to-day variability (more robust) | |
Disadvantages | • Chance of fluorescence bias due to the use of fluorescent dye | • Not capable of separating mRNA-LNP adducts | • Use of Expensive solvents |
• Not capable of separating mRNA-LNP adducts | • Sensitive towards matrix effects | ||
• Sensitive towards matrix effects | • Less sensitive than FA-Fluo | ||
• Use of CMR chemical and intercalant marker | • Complex device | ||
• Longer time required for sample preparation and analysis | |||
• Use of CMR chemical |
