Fig. 2

White light images of head and neck cancer tumor biopsies for each of the eight clearing process steps. The top and bottom rows show normal and fast clearing protocols, respectively. The time duration of each step is indicated: total time for normal and fast clearing is 18 h and 75 min, respectively. Abbreviations: Phosphate-buffered saline (PBS), Paraformaldehyde (PFA), Methanol (MeOH), Benzyl Alcohol Benzyl Benzoate (BABB).