Fig. 3

Quantitative analysis of tissue clearing effects. (A) Boxplots with median, interquartile range (IQR), minimum and maximum, showing the achieved transparency of both the tumor and healthy samples, including samples with and without tracer, for both tissue clearing protocols. Abbreviations: no significant difference (ns). (B) Boxplots with median, IQR, minimum and maximum measured fluorescence intensities (FI) relative to before clearing over a 28-day time period for the three approaches investigated: normal and fast clearing and a control. The solid dots represent outliers.