Fig. 5 | Scientific Reports

Fig. 5

From: Compatibility of fast tissue clearing with targeted fluorescence imaging and pathology analysis using clinical head and neck cancer biopsies

Fig. 5

Highlight showing the comparison between conventional fluorescence imaging and 3-dimensional (3D) confocal microscopy imaging following fast tissue clearing. For conventional fluorescence imaging, a detecting wavelength of 800 nm was used. For the 3D microscopy image, autofluorescence (green) channel was excited at 488 nm and detected at 550 ± 49 nm. The fluorescent tracer (red) channel was excited at 765 nm and detected at 795 ± 25 nm. Videos of additional 3D confocal microscopy examples are shown in the supplementary information.

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