Fig. 6 | Scientific Reports

Fig. 6

From: Protodioscin enhances Methotrexate-induced senescence and senolytic activity in HepG2 liver cancer spheroids by modulating cell cycle regulators and the TGF-β/p-Smad2-4 signaling pathway

Fig. 6

Live/dead assay for the viability of the HepG2 derived spheroids following treatment with Protodioscin (PD) and Methotrexate (MTX) for 24 h. Green fluorescence indicates Calcein AP stain in the live cells, and red fluorescence indicates the propium iodide stain in the dead cells. (A) Quantification from the live/dead assay using ImageJ software. An index of live cells (% of cell viability) was constructed from the ratio of live to total cell numbers. Data from three independent experiments were analyzed and presented as the mean ± SEM. Control (control group), MTX (6 μm), MTX + PD 1.6 (6 μm MTX + 1.6µM PD), MTX + PD 4 (6 μm MTX + 4µM PD) and MTX + PD 8.14 (6 μm MTX + 8.14µM PD).The results are reported as mean ± standard deviation**, and **** demonstrate significant difference between groups at P < 0.01 and p < 0.0001.

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