Fig. 7 | Scientific Reports

Fig. 7

From: Protodioscin enhances Methotrexate-induced senescence and senolytic activity in HepG2 liver cancer spheroids by modulating cell cycle regulators and the TGF-β/p-Smad2-4 signaling pathway

Fig. 7

The expression levels of p16 (A), p21 (B), p27 (C), and p53 (D) proteins in HepG2-derived spheroids following 24 h of treatment with Protodioscin (PD) and Methotrexate (MTX) were analyzed using the Western blot method. Control (control group), MTX (6 μm), MTX + PD 1.6 (6 μm MTX + 1.6µM PD), MTX + PD 4 (6 μm MTX + 4µM PD) and MTX + PD 8.14 (6 μm MTX + 8.14µM PD).GAPDH protein was used as a calibrator. The results are reported as mean ± standard deviation for tree replicates. *, **, *** and **** demonstrate significant difference between groups at P < 0.05, P < 0.01, p < 0.001 and p < 0.0001.

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