Table 1 Assay parameters for the determination of the studied analytes by the suggested method.

From: Towards sustainable pharmaceutical QC: micellar-UPLC strategy for concomitant analysis of a cardiovascular polypill with aniline and salicylic acid impurities

Analytes

Parameters

ASR

ATC

MES

RAP

SAA

ANL

Linearity range (µg/mL)

5–90

5–50

5–60

2–50

2–60

1–40

Correlation coefficient (r)

0.9998

0.9997

0.9998

0.9999

0.9999

0.9999

Slope

15.11

13.10

10.52

10.03

12.12

11.22

Intercept

−35.50

−26.73

−32.85

−4.02

−4.19

4.11

S.D of intercept a

6.10

4.53

3.34

1.18

2.16

1.04

LOD (µg/mL)

1.33

1.14

1.05

0.39

0.59

0.31

LOQ (µg/mL)

4.04

3.46

3.17

1.18

1.78

0.93

Accuracy

(Mean ± SD) b

99.16

±

1.23

101.03

±

1.11

98.15

±

1.34

99.85

±

1.05

99.20

±

0.95

100.34

±

1.07

Intra-day precision (RSD%) c

0.84

1.12

1.26

0.97

1.08

0.73

Inter-day precision (RSD%) d

1.03

1.19

1.45

1.20

1.16

0.97

  1. a Standard deviation of intercept.
  2. b Mean of five determinations.
  3. c Intra-day precision; relative standard deviation of (10, 40, and 80 µg/mL) of ASR, (5, 20, and 35 µg/mL) of ANL, and (10, 25, and 45 µg/mL) of ATC, MES, RAP, and SAA in triplicate within the day.
  4. d Inter-day precision; relative standard deviation of (10, 40, and 80 µg/mL) of ASR, (5, 20, and 35 µg/mL) of ANL, and (10, 25, and 45 µg/mL) of ATC, MES, RAP, and SAA in triplicate on three consecutive days.
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