Fig. 1 | Scientific Reports

Fig. 1

From: Generation and ophthalmological characterization of oculocutaneous albinism type 1 pig models by selection-free genome editing

Fig. 1

Production of TYR KO donor cells by selection-free genome editing. (A) Schematic overview detailing the process of generating OCA1 pigs using CRISPR-Cas9 RNPs and selection-free genome editing. (B) Evaluation of Cas9-mediated mutation frequencies for each sgRNA targeting TYR in JNPFF. (C) T7E1 assay for Cas9-mediated cleavage at TYR-targeting sites in each cell. (D) Examination of off-target editing frequency induced by Cas9-mediated gene editing in TYR KO donor cells.

Back to article page