Fig. 5
Comprehensive ophthalmological analysis of porcine OCA1 models. (A) Fundus images of WT and OCA1 pigs. (B) H&E staining of retinal sections from WT and OCA1 pigs sampled at 4 months of age. Abbreviations for the retinal layers: nerve fiber layer (NFL), ganglion cell layer (GCL), inner plexiform layer (IPL), inner nuclear layer (INL), outer plexiform layer (OPL), outer nuclear layer (ONL), photoreceptor inner/outer segments (IS/OS), retinal pigment layer (RPE), and epidermis (E). (C) IOP of the eyes of OCA1 pigs was higher compared with that of WT pigs. (D,E) Results for scotopic ERG (3.0 cd∙s/m2 light intensity) for the eyes of WT and OCA1 pigs. (D) Representative electroretinograms for WT and OCA1 pigs. (E) Comparison of b-wave amplitudes. (F–H) Results for OPs of the rising b-wave of scotopic ERG (3.0 cd∙s/m2 light intensity). (F) Representative graphs for OPs for WT and OCA1 pigs. (G) Comparison of the sum of OP amplitudes and (H) the ratio of the sum of OP amplitudes to b-wave amplitude between WT and OCA1 pigs. The in vivo clinical evaluations of the eyes of WT and OCA1 pigs at 10 months of age (n = 4 eyes from 2 pigs for each genotype). For bar plots, results are expressed as mean ± SD superimposed with data for individual eyes. Student’s t-test was used for comparison between groups; **p < 0.01, ns = not significant.
