Fig. 2 

Mutations of NTN1 and its receptors in pan-cancer and the 3D structure of important mutation sites. (a) The amino acid mutation of Netrin1 and its receptors in 32 types of TCGA cancer. The hot spot mutation is marked (Please check the edit and indicate how is the hot spot mutation marked, i.e., with what symbol or color). The substitution mutation is represented by a single-letter amino acid code separated by a slash. Other represents an important mutation site showing damaging in both VEST3 and REVEL algorithms and coverage < 0.1 in the functional and structural importance of protein-sequence positions. Phosphorylation representing the coincident site of phosphorylation and mutation. Transmembrane represents the transmembrane region. Caspase-3 represents the area that can be split by Caspase-3. (b) Binding site represents the amino acid mutation of Netrin and its receptor in CCLE, an important region that binds to Netrin1. Other (Please elaborate for clarity) indicates that it is mutated at both the CCLE and TCGA sites. (c) DCC hot spot mutation R661 mutation Q DSCAM 3D structure. (d) DSCAM hot spot mutation E368K/Q 3D structure. (e) The three-dimensional structure of the T861M mutation site of UNC5C (Swissmodel 3.0).