Fig. 1

Schematic overview of the analysis pipeline enabled by the dynamic constant infusion fPET protocol. The temporal structure of our data provides several novel avenues in context of studying metabolic changes in neurodegenerative diseases: metabolic activity within the time series can be analysed in a subject x time design, providing detailed molecular information per subject (a), within-subject variation in metabolic activity can be studied and related to group level and behavioral variables (b), seed-based connectivity can be studied on individual level based on metabolic time series, directly compared to fMRI connectivity maps and compared between groups (c). VC = coefficient of variation, fPET = functional positron emission tomography, HC = healthy controls, PD = Parkinson’s disease.