Fig. 7

Representative immunohistochemical staining of (A) RAGE; (B) p-p65; (C) IL-1β. Brown granules indicate positive expression of the respective proteins. For semi-quantitative analysis, the positive staining areas of (D) RAGE, (E) p-p65, and (F) IL-1β were quantified, and the results are presented as the percentage of positive staining area relative to the total tissue area. The sample size for each group was n = 6. All data are expressed as the mean ± standard deviation derived from three independent experiments. Statistical analyses were performed using one-way ANOVA followed by Tukey’s multiple comparisons test. *P < 0.05; ***P < 0.001.