Fig. 1
From: Decellularized lymph node sections with preserved extracellular matrix for stromal cell culture
Lymph node sectioning and decellularization schematic and workflow. (A) Murine and human lymph nodes were harvested, embedded in agarose, sectioned into 200 μm slices using a vibratome, and decellularized with SDS and Triton-X. The dLN sections were then seeded with FRCs, cultured for 1–28 days, and subsequently harvested using an enzymatic digestion mix for further downstream analysis. (B) Dissected lymph nodes were embedded in 6% agarose and allowed to solidify. Once set, samples are cored using a 6-mm (mouse) or 8-mm (human) biopsy punch and mounted into the vibratome disc. Lymph nodes were sectioned into 200 μm sections using a vibratome. The resulting sections were then decellularized.
