Fig. 6
From: Decellularized lymph node sections with preserved extracellular matrix for stromal cell culture
Human validation of decellularized lymph node sections. Human lymph nodes were sectioned (-/-), decellularized (+ /-), and seeded (+ / +) with human FRCs. (A) DNA concentration in control and decellularized human lymph nodes (n = 3 human donors). (B) Bioluminescence longitudinal assessment of human FRC viability via RealTime-Glo™ assay for 21 days (n = 5 human dLN sections seeded with 30,000 human FRCs per dLN section). (C) Representative immunofluorescence confocal images of control non-decellularized LN section (-/-), dLN sections before cell re-seeding (+ /-), and dLN sections seeded with human FRCs (+ / +) and cultured for 14 days stained for DAPI (Nuclei, gray), Collagen I (magenta), and α-SMA (green). Scale bar = 100 μm. Decell = Decellularized; FRCs = Seeded with FRCs. (D) Representative immunofluorescence confocal images of control non-decellularized LN sections (-/-) and dLN sections seeded with human FRCs (+ / +) and cultured for 14 days stained for DAPI (Nuclei, gray) and F-actin (red). Scale bar = 100 μm. Decell = Decellularized; FRCs = Seeded with FRCs. Data represented as mean ± standard deviation. P-values were determined by unpaired, student’s t-test (*p < 0.01).
