Fig. 4
Layer-specific TMPRSS2 localization in human hair follicles revealed by co-staining with KRT6/75. TMPRSS2 (green) expression in longitudinal (A–A″) and cross (B–B′) sections of human hair follicles (HFs). Single TMPRSS2 (green) expression in a longitudinal (A–A’’) section of plucked HFs. The highlighted magnified section in (A’) shows the expression of TMPRSS2 in the area of the outer layer of the ORS. The highlighted magnified section in (A’’) shows the expression of TMPRSS2 in the area of the hair bulb. There is a clear expression in the outermost layer of the hair bulb. Co-staining of TMPRSS2 (green) and ORS/companion layer-specific marker KRT6/75 (red) in a cross (B) section of plucked HFs. The highlighted magnified section in (B’) shows the expression of TMPRSS2 in the He, which is a part of the IRS and directly connects to the companion layer (CP) inwards. Nuclear marker DAPI is shown in blue. Scale bar 200 μm (A), 50 μm (A’, A’’, B), 10 μm (B’).
