Fig. 1
From: Unbiased assessment of 2-arachidonoylglycerol in cardiovascular inflammation
2-AG induces transcriptomic changes in human monocytes with pro-inflammatory features. (A) Shema of in vitro culture of human monocytes isolated from buffy coats of healthy donors. Monocytes are cultured in presence and absence of 2-AG for up to 24 h. (B) Bar charts of flow cytometry analysis of the endocannabinoid receptors CB1 and CB2 on human monocytes which serve as receptors for 2-AG. (C) Principle component analysis of the transcriptomes of monocytes treated with 2-AG and respective controls. (D) Overview on the number of differentially expressed (DE) genes identified by 2way-ANOVA with a fold-change of |1.5| and p-Value < 0.05. (E) Heatmap of 416 genes which were significantly up-regulated in its expression by 2-AG. Genes were categorized in to clusters according their GO (gene ontology) term categorization. (F) Dot plot for the 270 2-AG induced genes belonging to the GO term “Regulation of response to stimulus”. Gene of the top 2-AG induced surface markers are marked in the plot. (G) Bar chart of the top 5 enriched hallmark gene sets in transcriptomic data sets of human monocytes cultured for 24 h in the presence of 2-AG compared to monocytes cultured for 24 h without 2-AG. (n = 3–4).
