Fig. 1
RP11 is upregulated in ovarian cancer cells and promotes their proliferation. (A) RP11 expression in HGSOC tissues and normal fallopian tube tissues was compared via qRT‒PCR. (B) The two most appropriate cell lines were selected from among the five cell lines for subsequent experiments. (C) Fluorescent protein expression after cells were transduced with lentivirus for 72 h (scale bars, 1000 µm). (D) RP11 expression in cells transduced with the LV-RP11 lentivirus significantly increased. (E) RP11 expression after RP11 knockdown via lentivirus. (F,G) In OVCAR3 and HO8910 cells, RP11 overexpression promoted cell proliferation, and RP11 silencing suppressed cell proliferation. H: Colony formation increased in ovarian cancer cells overexpressing RP11. I: RP11 knockdown inhibited colony formation. (**P < 0.01; ***P < 0.001; ****P < 0.0001). Data are presented as mean ± SD with 95% confidence intervals for pairwise differences; Bonferroni post-hoc correction was applied and adjusted P values are indicated in the figure.
