Fig. 7
Expression pattern of R72A10-GAL4 in the adult Drosophila central nervous system and lack of Octopaminergic Identity. Confocal images show UAS-mCD8::GFP expression driven by R72A10-GAL4 in the central brain (a1–a3) and ventral nerve cord (b1–b3) of an adult male fly. R72A10-labeled neurons (green) are visualized using α-mCD8 staining, and neuropil regions are counterstained with α-NC82 (gray). In the central brain (a1, a3), labeled neurons are distributed across several regions, including the antennal lobes (AL), subesophageal ganglion (SOG), and lateral protocerebral complex. A cluster of ~ 10–15 neurons is consistently observed at the AL–SOG junction (yellow rectangle), with cell bodies located at this junction and projections extending toward higher brain regions (a3). Additionally, approximately 10 -12 neurons were labeled in the ventral nerve cord (b1-b3).To examine neurotransmitter identity, brains of R72A10-GAL4/UAS-mCD8::GFP flies were co-labeled with α-GFP (green) to mark R72A10 neurons (c1) and α-Tdc2 (magenta) to mark octopaminergic neurons (c2). No overlap was detected in the merged image (c3), suggesting the neurons are not octopaminergic. The yellow rectangle highlights the ~ 10–15 neurons at the AL–SOG junction (c3). Scale bars, 25 µm.
