Fig. 2

Saccharomyces cerevisiae samples prepared identically and imaged in a synchrotron-based and in a lab-based SXT microscope. (A) Reconstruction slices through a tomogram acquired at U41-XM beamline at BESSY-II synchrotron, (B) Intensity profile and a full width and half maximum (FWHM) measurement across the vacuole membrane in a synchrotron-based tomogram reconstruction slice. (C) Reconstruction slices through a tomogram acquired at the lab-based microscope, (D) Intensity profile and a FWHM measurement across the vacuole membrane show in a lab-based tomogram reconstruction slice.