Fig. 5 | Scientific Reports

Fig. 5

From: Demonstrating soft X-ray tomography in the lab for correlative cryogenic biological imaging using X-rays and light microscopy

Fig. 5

Euglena gracilis cells cryo-vitrified on 3 mm Quantifoil-coated TEM grids and imaged by cryo-SXT and the integrated fluorescence microscope in the SiriusXT table-top SXT microscope. (A) A visible light fluorescence mosaic of a portion of a 3 mm TEM grid showing grid windows and the chlorophyll fluorescence signal. The area where the soft X-ray tomogram was collected, as shown in panel B, is enclosed in a green rectangle. (B) A composite FM image of the E. gracilis chloroplast overlaid with a low magnification X-ray projection image acquired with 10 s exposure and a virtual slice through a high magnification SXT tomogram acquired over 1.5 h. (C–E) Virtual slices through tomograms with 29 × 29 µm2 field of view featuring seven E. gracilis cells.

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