Fig. 6
Saccharomyces cerevisiae yeast cells imaged using the lab-based microscope. (A) A portion of a visible light FM images mosaic of a 3 mm TEM grid with vitrified yeast cells labelled with BODIPY493/503 dye. (B) a single fluorescence image of a grid area containing a number of yeast cells. (C) A mosaic of low magnification X-ray transmission images covering an area of 370 × 350 µm2 acquired in less than 5 min. (D) A single low magnification X-ray transmission image capturing yeast cells within a 43 × 58 μm² field of view on a grid window acquired with a 10-second exposure. (E) Zero-tilt X-ray tomogram projection displaying a 30 × 30 μm field of view overlaid with a MSSR-processed fluorescence image. (F) A virtual slice extracted from the reconstructed tomogram acquired over one hour.
