Fig. 1 | Scientific Reports

Fig. 1

From: Prognostic value of CD28⁻CD57⁺CD8⁺ T cells for early immunotherapy response in hepatocellular carcinoma: a prospective observational study

Fig. 1

Representative flow cytometry gating strategy and analysis of peripheral blood. (A1-A4) Gating sequence for a patient with a high baseline proportion of CD28⁻CD57⁺CD8⁺ T cells. (B1-B4) Gating sequence for a patient with a low baseline proportion of CD28⁻CD57⁺CD8⁺ T cells. ① (A1, B1): Single cells were gated from all acquired events using FSC-A versus FSC-H to exclude doublets and aggregates. ② (A2, B2): Lymphocytes were identified and gated from the single-cell population based on CD45 expression and side scatter (SSC-A) properties. ③ (A3, B3): CD3⁺CD8⁺ T cells were gated from the lymphocyte population. ④ (A4, B4): The expression of CD28 and CD57 was analyzed on the CD3⁺CD8⁺ T-cell population (P3). The proportion of CD28⁻CD57⁺ cells (quadrant Q1-UL) is indicated and represents the senescent T-cell subset of interest. FSC, forward scatter; SSC, side scatter.

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