Fig. 4 | Scientific Reports

Fig. 4

From: Sodium butyrate-induced autophagy in colorectal cancer unveils the Ca²⁺/CaMKKβ signaling pathway as a potential therapeutic target

Fig. 4

Reducing CaMKKβ expression via RNA interference decreased NaB-mediated autophagy in colorectal cancer cells. (A) The HT29 cell line was exposed to either a non-targeting control sequence or one of two specific interference sequences for a duration of 24 h. Thereafter, alterations in CaMKKβ mRNA expression levels were quantified through quantitative reverse transcription polymerase chain reaction (RT-PCR). (B) Following CaMKKβ knockdown via RNA interference, HT29 cells were treated with 2 mM NaB for either 24 h2 h. Western blotting with densitometric quantification was subsequently performed: the 24 h group was used to assess LC3II levels, whereas the 2 h group was analyzed for AMPKα, ACC, p-CaMKKβ, p-AMPKα, and p-ACC expression. (C) Quantifying LC3 mRNA levels was done by quantitative RT-PCR. *P < 0.05, **P < 0.01, ***P < 0.001.

Back to article page