Fig. 1

Multiple sequence alignment of EstN3 and selected family VIII.2 esterases (Selected Region only). Multiple sequence alignment was performed with Clustal Omega (1.2.4) and visualized with ESPript 3.0. Conserved residues are shaded in red (0.7 Similarity threshold). The catalytic Ser and Lys are marked with an arrow. The number at the beginning represents the position of the amino acid in the sequence. The abbreviations and accession numbers of proteins used in this analysis are as follows: EstCE1 [uncultured bacterium pCosCE1] (AAY90130.1), EstBL [Burkholderia cepacia] (AAX78516.1), Est22 [uncultured bacterium] (AGT17593.1), EstF4K [uncultured microorganism] (AEH57832.1), EstM-N2 [uncultured bacterium] (AEA07655.1), EstG34 [uncultured microorganism] (AIL90340.1).