Table 1 Effect of different inhibitors on EstN3’s activity: assays were carried out by incubating the protein with three inhibitor concentrations (0.1 mM, 1 mM, and 5 mM). Activity without an inhibitor was taken as 100%. All reactions were carried out in triplicate. The residual activity percentage is presented as a mean value with standard deviation. ^a:p-chloromercuriobenzoate, ^b: mercuric chloride, ^c: diethyl pyrocarbonate, ^d: phenylmethylsulfonyl fluoride, ^e: N-ethylmaleimide.

From: Discovery and characterization of an enantioselective family VIII esterase from effluent treatment plant sludge metagenome