Fig. 2
From: TIR-domain-containing protein C as modulator of innate immune checkpoints
TcpC enforces CFT073 induced TLR4- and MyD88-dependent cytokine secretion by the human bladder epithelial cell line T24/83. Wild type (A,D), myd88−/− (B,E), and tlr4−/− human bladder epithelial T24/83 cells (C,F) were infected for 5 h with CFT073, CFT073ΔtcpC or CFT073ΔtcpC + pTcpC with different MOIs as indicated. IL-6 (A–C) and TNFα levels (D–F) were determined in the culture supernatant. LPS + ATP served as positive, medium as negative control. The experiments were repeated twice with similar results, each bar represents three replicates. **P < 0.01, ***P < 0.001, ****P < 0.0001, two-way ANOVA, post hoc Tukey.
