Fig. 7
From: TIR-domain-containing protein C as modulator of innate immune checkpoints
TcpC impairs bacterial replication nevertheless stimulates monocytic THP-1 cells. We induced CFT073ΔtcpC + pASK-IBA5-TcpC with titrated doses of Atc and analyzed the cytosolic expression of TcpC by Western blot. We also used CFT073 to explore endogenous expression levels of TcpC. The observed size difference of TcpC expressed by CFT073 versus CFT073ΔtcpC + pASK-IBA5-TcpC is due to an inserted Strep-tag in the latter case (A). The effect of TcpC induction on bacterial replication is depicted in (B), each bar represents one culture. We infected monocytic THP-1 cells with CFT073, CFT073ΔtcpC, or CFT073ΔtcpC + pASK-IBA5-TcpC (MOI = 0.1), whose TcpC expression was induced with titrated amounts of Atc. We determined IL-1β (C) and TNFα (D) in culture supernatants. The experiment depicted in (C) and (D) was performed with three replicates and repeated twice with similar results. We monitored bacterial replication bevor (E) and after infection of THP-1 cells (F). Graphs in (E) and (F) depict three replicates. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, one-way ANOVA, post hoc Tukey.
