Fig. 8
From: TIR-domain-containing protein C as modulator of innate immune checkpoints
Transfer of CFT073ΔtcpC + pASK-IBA5plus-TcpC culture supernatants reduced cytokine secretion by monocytic THP-1 cells. We stimulated monocytic THP-1 cells with culture supernatants of CFT073ΔtcpC + pASC-IBA5-TcpC, whose TcpC expression was not or induced with titrated amounts of Atc as indicated. The supernatants were concentrated using a 10 kD cut off filter to remove consumed culture medium and re-diluted with fresh culture medium before they were added to the cell culture. Monocytic THP-1 cells were not or were additionally stimulated with endotoxin plus ATP as indicated. We measured TNFα (A,B) and IL-1β (C,D) in culture supernatants. Endotoxin plus ATP +/− Atc served as positive, medium +/- Atc as negative control (B,D). Graphs depict three replicates. The experiment was repeated with similar results. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, one-way ANOVA, post hoc Tukey.
