Fig. 4 | Scientific Reports

Fig. 4

From: Rational design and in silico characterization of a multiepitope mRNA vaccine candidate against human metapneumovirus (hMPV) using reverse vaccinology and immunoinformatics approaches

Fig. 4

The predicted three-dimensional structure of the screened vaccine construct (hMPVbeta 1) and validation of the 3-D structure: (a)-Robetta-predicted and Galaxy-refined 3-D model of the hMPVbeta1 construct, (b)-Prosa-web-based validation plot with z-score (c)-Ramachandran plot, and (d)-energy validation plot of a 3-D model of the hMPVbeta 1 construct. The light blue region in section b of the figure corresponds to high-quality protein structures solved using X-ray crystallography, and the dark blue region represents high-quality structures solved using NMR. The two lines represent the energy calculated with different window sizes (10 and 40) in section d of the figure, which smooth out the fluctuations over short or long sequence segments. RF = Ramachandran favored FR = Favoured region, and AR = allowed region.

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