Table 2 The Immunogenic potential of the prioritized promiscuous MHC-class-2 binders (IL-4 inducer overlapped) and one IFN-gamma epitopes (IL-4 inducer overlapped).

From: Rational design and in silico characterization of a multiepitope mRNA vaccine candidate against human metapneumovirus (hMPV) using reverse vaccinology and immunoinformatics approaches

Proteins IDs/Proteins

HTL -epitope designation

HTL-epitope/allele pair

IC50-value

Ag(s)

II/IL-4 score

Tox/Aller

YP_009513268.1/hMPV-Fp

HTL − 1

ADLKMAVSFSQFNRR/HLA-DRB3*02:02

96.6

1.22

II/1.32

NT/NA

HTL − 2

NINISTTNYPCKVST/HLA-DRB1*13:02

89.8

1.0

II/1.31

NT/NA

YP_009513272.1/hMPV-Gp

HTL − 3

RSKCFKNASLVLIGI/HLA-DRB3*02:02

16.4

1.187

II/0.23

NT/NA

HTL − 4

IRTIDMLKARVKNRV/HLA-DRB5*01:01

4.3

1.055

II/0.3

NT/NA

MH828686.1/hMPV-Mp

HTL − 5

KCFKNASLVLIGITT/HLA-DRB1*01:01

61.3

1.164

II/0.21

NT/NA

HTL − 6

RSKCFKNASLVLIGI/HLA-DRB3*02:02

16.4

1.187

II/0.23

NT/NA

YP_009513271.1/hMPV-Shp

HTL − 7

TYKFLCFKSEDTKTN/HLA-DRB1*01:01

34.2

1.01

II/0.28

NT/NA

HTL − 8

TCWKINRNQCTNITT/

HLA-DRB1*13:02

31.9

0.52

II/0.27

NT/NA

sProteins IDs/Proteins

IFN-γ -epitope designation

IFN-gamma Epitope/allele pair

IC50-value

Ag(s)

IFN-γ/IL-4 scores

Tox/Aller

YP_009513268.1/hMPV-Fp

IFN-γ −1

EGEQHVIKGRPVSSS/

HLA-DRB1*01:01

49.6

0.4351

1.0/1.07

NT/NA

  1. hMPV = human metapneumovirus, Fp = fusion protein, Mp = matrix protein, Gp = attachment glycoprotein, Shp = short hydrophobic protein, IFN-γ = interferon-gamma, HTL = helper T-cell, Ag(s) = antigenicity score (Vaxijen), IL-4 = interleukin-r, II = IL-4 inducing, Tox = toxicity, Aller = allergenicity, NT = non-toxic, and NA = non-allergenic.
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