Table 3 Antigenic, allergenic, toxigenic, and physiochemical characteristics of the target four models of vaccine construct.

From: Rational design and in silico characterization of a multiepitope mRNA vaccine candidate against human metapneumovirus (hMPV) using reverse vaccinology and immunoinformatics approaches

Parameters

hMPV-

beta1

hMPV-

beta2

hMPV-Ribo1

hMPV-Ribo2

Allergenicity

Non-allergen

Non-allergen

Non-allergen

Non-allergen

Antigenicity/score

Ag/0.746

Ag/0.7322

Ag/0.6662

Ag/0.658

Residue toxicity/ToxinPred 2.0 score

Non-toxic/0.21

Non-toxic/0.25

Non-toxic/−0.3

Non-toxic/−0.33

AA Length

383

385

468

470

Mw/TpI

39633.71

/9.92

39668.16

/9.84

47913.00

/9.35

47947.45

/9.19

AI

68.75

67.53

78.63

77.60

GRAVY

−0.260

−0.429

−0.095

−0.234

I-i

31.24

30.59

26.86

26.36

EHL-MR

1 h

1 h

1 h

1 h

EHL-Y

30 min.

30 min.

30 min.

30 min.

EHL-E

> 10 h

> 10 h

> 10 h

> 10 h

EC (M−1cm−1)

39,475

36,495

35,870

32,890

E. solubility

0.980

0.904

0.749

0.686

E. solubility/soluscore

SE/0.784

SE/0.901

LSE/0.416

LSE/0.53

  1. hMPV = human metapneumovirus, AA = amino acid, N-Ag = non-antigenic, Ag = Antigenic, Mw = molecular weight, Tpi = theoretical pI, pI = isoelectric point, AI = aliphatic index, grand average of hydropathicity, Ii = instability index, EHL-MR = estimated half-life in mammalian reticulocytes or invitro, EHL-Y = estimated half-life in Yest or in vivo, EHL-E = estimated half-life in E. coli or in vivo, E. solubility = expression solubility, SE = soluble expression, LSE = lower soluble expression, suloscore cut off > 0.5, and EC = extinction coefficient measured in water at 280.
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