Fig. 4 | Scientific Reports

Fig. 4

From: Disulfiram inhibits Gasdermin D pores formation and improves insulin-dependent glucose uptake and glucose homeostasis in skeletal muscle of obesity-induced insulin-resistant mice

Fig. 4

Disulfiram promotes insulin-dependent Akt phosphorylation and increases glucose uptake in FDB muscle fibers in insulin-resistant mice. Ratio of phosphorylated to total Akt (P-Akt/Akt) in FDB and 2-NBDG uptake assay in FDB muscle fibers. a Representative Western blot assay and quantification of P-Akt protein levels in muscle homogenates (n = 4). Total Akt was used as a loading control. b Representative epifluorescence images (green fluorescence) and quantification of 2-NBDG uptake (n = 4 independent experiments). 2-NBDG was used at 300 µM; insulin at 100 nM for 20 minutes. (Scale bar = 50 µm). For the analysis of four groups, a two-way ANOVA with Tukey’s multiple comparisons test was performed. Results are presented as mean ± SEM and expressed relative to the HFD group. * p < 0.05, **p < 0.01, and ****p< 0.0001 vs. HFD. HFD: High-fat diet, HFD+DSF: High-fat diet injected with 50 mg/kg three times per week for 3 weeks. FDB: flexor digitorum brevis.

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