Fig. 4 | Scientific Reports

Fig. 4

From: Treadmill exercise ameliorates atherogenesis and vascular inflammation in ApoE−/− mice via circulating exosome-derived let-7c-5p

Fig. 4

let-7c-5p negatively regulates Timp-3 expression. (A) Representative images of exosomes captured by transmission electron microscopy and NTA analysis showing the particle size distribution of serum-derived exosomes from two groups. (B) Exosome biomarkers include CD9, CD63, and TSG101 examined via Western blot analysis. (C) Results of the let-7 family expression by high-throughput sequencing. MiRNAs with TPM (Transcripts per million) values less than 1 were excluded. (D) Relative expression of let-7 family members in the aortas via qRT-PCR (n = 5–7 per group). The fold change was calculated based on delta Ct between endogenous U6 control. (E) Dual-luciferase assay to demonstrate the interaction between Timp-3 and let-7c-5p (n = 5 per group). *P < 0.05, **P < 0.01, ***P < 0.001, ns indicating P > 0.05.

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