Fig. 7 | Scientific Reports

Fig. 7

From: Treadmill exercise ameliorates atherogenesis and vascular inflammation in ApoE−/− mice via circulating exosome-derived let-7c-5p

Fig. 7

let-7c-5p ameliorates inflammation by regulating the expression of Timp-3. (A) The overexpression and knockdown efficiency of Timp-3 were verified by qRT-PCR analyses (n = 5 per group). si-Timp-3, siRNA for Timp-3 knockdown. pCDNA3.1-Timp-3 for Timp-3 overexpression. (B) MOVAS cells were transfected with si-Timp-3 or pCDNA3.1-Timp-3 and induced with ox-LDL, followed by qRT-PCR validation of MMP-9 expression (n = 5 per group). ox-LDL, oxidized low-density lipoprotein. (C–E) MOVAS cells were transfected with let-7c-5p mimics or let-7c-5p inhibitor and induced with ox-LDL, followed by qRT-PCR validation of Timp-3 (C), IL-6 (D), and TNF-α expression (E) (n = 5 per group). (F) Quantification of rescue experiments. (G) The schematic diagram illustrates the mechanism of treadmill exercise in stabilizing plaques and ameliorating atherogenesis. Treadmill exercise changes circulating exosomal miRNAs, reduces the level of let-7c-5p in serum and aorta, which increases the expression level of Timp-3, thus playing a role in alleviating atherosclerotic inflammation. The diagram was drawn by Figdraw (www.figdraw.com). *P < 0.05, **P < 0.01, ***P < 0.001, ns indicating P > 0.05.

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