Fig. 3 | Scientific Reports

Fig. 3

From: dFlpTag, a RMCE-based tool for simultaneous endogenous protein tagging and cell labeling in Drosophila

Fig. 3

Conditional Brp tagging using the dFlpTag system. Conditional GFSTF and smGFP::HA tagging strains were generated by swapping BrpMI02987. (A) In larval VNCs, neuropils were specifically labeled by anti-GFP staining in the BrpdFlpTag−GFSTF(cond.) strain, or by anti-HA staining in the BrpdFlpTag−smGFP::HA(cond.) strain when UAS-FLP was expressed by pan-neuronal R57C10-GAL4. Neurons were also labeled by mtdTomato in these samples. (B) R13F02-GAL4 was used to express UAS-FLP in the Kenyon cells (KCs) of the mushroom body (MB). In adult brains of BrpdFlpTag−smGFP::HA(cond.) strain, FLP activated GAL4-driven mtdTamato expression for labeling the entire MB, while HA-tagged Brp was preferentially detected in KC axons (arrows) but not in KC dendrites (arrowheads) or soma (double arrows). Scale bars: 20 μm.

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