Fig. 5
From: AFP promotes cancer multidrug resistance through activating PI3K/Akt/NF-κB signaling pathway
The influence of AFP on regulating RELA(p65)-mediated MDR and anti-apoptotic gene expression in HCC and cervical cancer cells. A: (a) Spearman correlation analysis of two genes, RELA and ABCB1, RELA and ABCC1, RELA and ABCG2, RELA and BIRC2, RELA and BIRC7, and RELA and XIAP in HCC. (b) Spearman correlation analysis of multiple genes, RELA and ABCB1, ABCC1, ABCG2, BIRC2, BIRC7, and XIAP in HCC. (c) Bel7402, Bel7402-shNC, and Bel7402-shAFP cells were treated with PI3K inhibitor LY294002 for 12 h, Western blotting was used to detect the changes in the expression of various drug resistance genes. B: (a) Spearman correlation analysis of two genes, RELA and ABCB1, RELA and ABCC1, RELA and ABCG2, RELA and BIRC2, RELA and BIRC7, and RELA and XIAP in cervical cancer. (b) Spearman correlation analysis of multiple genes, RELA and ABCB1, ABCC1, ABCG2, BIRC2, BIRC7, and XIAP in cervical cancer. (c) HeLa, HeLa-NC, and HeLa-AFP cells were treated with PI3K inhibitor LY294002 for 12 h, Western blotting was used to detect the changes in the expression of various drug resistance genes. The results represented in the figure are derived from three independent experiments (n = 3). **P < 0.01.
