Fig. 6

HZ effect on dTHP-1 polarized with M1 stimuli used for MDM. M1 macrophage polarization markers assessed as mRNA expression levels (A) and protein secretion levels in cell supernatants (B) in dTHP-1 macrophages after 24 h incubation with HZ and M1-polarizing stimuli used for MDM (IFN-γ + TNF-α). Gene expression of CXCL8, IL1B and IL6 was evaluated by Real-Time PCR analysis. Results are expressed as fold change calculated by the ΔΔCt method. Data are presented as mean ± standard deviation from three independent experiments for dTHP-1. CXCL8, IL-1β and IL-6 protein levels were measured by ELISA and expressed as % of the control, with data represented as the mean ± standard deviation. Statistical analysis was performed using an unpaired two-tailed t-test for each gene or protein, comparing the data to the control (M1-stimulated macrophages). Statistical significance is denoted as follows: *p < 0.05.