Fig. 7

Effect of HZ on M2 macrophages. Macrophage polarization markers evaluated as mRNA expression levels (panels A,C) and protein secretion in the supernatants (panels B,D) in MDM (filled columns—panels A,B) and dTHP-1 cells (striped columns—panels C,D) after 24 h of incubation with HZ and M2-polarizing stimuli. Gene expression of CXCL8, TNFA, IL1B, IL6, IL10 and PPARG was evaluated by Real-Time PCR analysis. Results are expressed as fold change calculated by the ΔΔCt method. Data are presented as mean ± standard deviation from four different donors for MDM and from at least three independent experiments for dTHP-1. CXCL8, TNF-α, IL-1β, IL-6 and IL-10 protein levels were measured by ELISA and expressed as % of the control, with data represented as the mean ± standard deviation. Statistical analysis was performed using an unpaired two-tailed t-test for each gene or protein and comparing the data versus the control (M2-stimulated macrophages). Statistical significance is denoted as follows: *p < 0.05; **p < 0.01.